Alternative transcripts in DNA repair genes are induced by IR.

<p>Alternative transcripts in the DNA repair genes, XPC (A) and RRM2B (B) in response to IR are shown. Graph axes are as in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053358#pone-0053358-g001" target="_blank">figure 1</a>; PCR products from 5′-RML-RACE were run on a 2% agarose gel. An arrow indicates the amplicon from the alternative initiated transcript that was sequenced (gel picture to the right of the panel; <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053358#pone.0053358.s004" target="_blank">Figure S4</a>). Diagrams of the predominant transcripts (initiating by P1) and the alternatively initiated (P2) transcripts after IR are shown below. Primer locations for 5′ RLM-RACE are indicated below exon 2 (arrow pointing to the left).</p>