Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy-4

SIV mac239 and SIV PBJ. Numbers indicate residues on the first amino acids of the shown sequences. Colored residues in HIV-1 Tat were mutated in this study. CHME5 cells were cotransfected with a plasmid encoding GFP and constructs expressing the first exon of HIV-1 Tat (psvTat72), SIV-PBJ Tat, or with an empty plasmid (pcDNA3.1) using Lipofectamine. Cells were then treated with LPS/CHX and analyzed for cell death. Bright fields (BF) and merged (red+green) fields are shown. Transfected cells are GFP+ cells (green), dead cells (red). The percentage of cell death induced in GFP+, EthD+ cells is shown with the SD from three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Akt inhibitors as an HIV-1 infected macrophage-specific anti-viral therapy"</p><p>http://www.retrovirology.com/content/5/1/11</p><p>Retrovirology 2008;5():11-11.</p><p>Published online 31 Jan 2008</p><p>PMCID:PMC2265748.</p><p></p>