Adipogenic changes in the growth plate chondrocytes transfected with a PPAR plasmid under the control of a collagen 1(II) promoter
2011-12-31T03:21:50Z (GMT) by
Taken from "Peroxisome Proliferator-Activated Receptor-γ Promotes Adipogenic Changes in Growth Plate Chondrocytes In Vitro"
PPAR Research 2006;2006():-.
Published online 20 Sep 2006
PMCID:PMC1779577.(a) Growth plate cells were transfected with the p1757-PPAR plasmid. Transfected cells were maintained in pellet cultures for 10 days, followed by Oil Red-O staining. (b) Quantitative PCR analysis of adipogenic markers of the p1757-PPAR transfected growth plate cells at day 4 after transfection. Cells transfected with the empty p1757 plasmid were used as controls. Gene expression levels were normalized with respect to endogenous 18S rRNA. * < .05 versus the expression in control cells.