Additional file 9: of Regulation of constitutive and alternative mRNA splicing across the human transcriptome by PRPF8 is determined by 5′ splice site strength

Transcription initiation is similar in control siRNA-treated and PRPF8-depleted cells. a Delay between detection of the unspliced nascent transcript and the partially spliced transcript for UBE2R2 is similar in both PRPF8-depleted and control siRNA-treated cells. Kinetics of transcription and splicing recovery for UBE2R2, a gene whose splicing was unaffected by PRPF8 depletion as determined in our RNA-sequencing data, following release from drug-induced transcriptional arrest were measured in control siRNA-treated and PRPF8-depleted cells in a similar fashion to that shown in Fig. 8d. b Transcription of the first exon–intron junction of the four target genes following release from DRB in PRPF8-depleted and control siRNA-treated cells was measured. Primer pairs used span exon 1: intron 1 and thus measure transcription initiation following release from drug-induced transcriptional arrest. (PDF 198 kb)