Additional file 9: Figure S8. of HSP90 promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance via Thr-328 phosphorylation in hepatocellular carcinoma

Thr-328 phosphorylation-induced by HSP90 overexpression was critical for maintaining the function of PKM2. In Huh7 cells with endogenous PKM2 depleted, transfection of T328A PKM2 mutant significantly reduced (A) glucose consumption, (B) lactate production and (C) PK catalytic activity of Huh7 cells. Furthermore, transfection of T328A PKM2 mutant significantly reduced (D) proliferation and increased the (E) Caspase-3 activity and (F) apoptosis the of Huh7 cells. (TIFF 1030 kb)