Additional file 8: of Identification of enhanced hydrogen and ethanol Escherichia coli producer strains in a glycerol-based medium by screening in single-knock out mutant collections
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Table S2. The primers from 1 to 6 were used to obtain multiple mutants; ldhAgnd:kan (M2), ldhAgndfrdBC::kan (M4) and ldhAgndfrdBCtdcE::kan (M5). Capital letters indicate the homologous sequences of the target genes and in lower case letters indicate the priming sequences of the pKD13 vector. In the names of the primers, H2P1 indicates the forward primers and H1P4 the reverse primers. Primers from 7 to 15 were used for PCR verification of the deletion of the targeted genes.