Additional file 8: Figure S8. of IMPACT is a GCN2 inhibitor that limits lifespan in Caenorhabditis elegans

SKN-1 and DAF-16 target gene expression upon impt-1 RNAi. (a) Average integrated density of GFP expression of the Pgst-4::GFP reporter at different larval stages [L1 (L4440, n = 19; impt-1, n = 20), L2 (L4440, n = 36; impt-1, n = 29), L4 (L4440, n = 23; impt-1, n = 31); Student t test). This is a composite of two independent experiments. (b) Representative images of Pgst-4::GFP expression in L2 larvae treated with L4440 (control) or impt-1 RNAi from L1. Dashed lines delimitate the body of the worm. Head and tail are indicated by arrows. (c) Average integrated density of GFP expression of the Psod-3::GFP reporter at different larval stages [L1 (L4440, n = 9; impt-1, n = 12), L2 (L4440, n = 18; impt-1, n = 24), L4 (L4440, n = 14; impt-1, n = 12); *P < 0.05, Two-way ANOVA, Sidak post-hoc]. This is composite of two independent experiments. Bars are mean ± SEM. (PDF 390 kb)