Additional file 7: Figure S7. of Expression and functional analysis of the Wnt/beta-catenin induced mir-135a-2 locus in embryonic forebrain development

Early mir-135a-2 overexpression affects hippocampus development. (A-D) Coronal sections of post-natal stage P1 and adult brains processed for ish for NeuroD2. Panels A, B show overall morphology and size of the hippocampus. Asterisks point towards the pallium-subpallium boundary. Insets show the extent of the hippocampus from the CA1 field to the tip of the dentate gyrus, quantification of which, at mid and caudal level of the brain, is reported in panel E. Length is expressed in μm ± SEM (n = 4). Consistent with a reduced cortical hem size at embryonic stages, the hippocampus was significantly reduced in its extent in mutant brains with respect to controls. ***, p <0.001. Abbreviations: pSub, para subiculum; Sub, subiculum; DG, dentate gyrus; Cng, cingulate cortex. Scale bar 400 μm in panels A and B; 200 μm in panels C and D. (TIF 2162 kb)