Additional file 7: Figure S6. of Patient-derived heavy chain antibody targets cell surface HSP90 on breast tumors

Calnexin knockdown did not lead to increased HCAb2 binding to MDA-MB-231 cells. A. Immunoblot analysis of MDA-MB-231 cells transiently transfected with mock (transfection medium alone) or luciferase siRNA (siLuc) or calnexin siRNA (siCANX) at different concentrations. β-actin was used as the loading control. B. Flow cytometry analysis of mock, siLuc (25 nM) or siCANX (25 nM) transfected MDA-MB-231 cells using HCAb2. Percentages of cells that showed positive binding with HCAb2 in comparison to isotype control are shown in the histograms. Unstained (blue peak), isotype control (red peak) and HCAb2 (yellow peak). (TIFF 1523 kb)