Additional file 7: Figure S6. of Nascent osteoblast matrix inhibits osteogenesis of human mesenchymal stem cells in vitro

Comparison of the matrix effect of human foreskin fibroblasts and osteoblastic cells on osteogenic differentiation of MSCs. HFF and OBC cultures were water-lysed and the resultant matrices used as a substrate for MSCs cultures maintained in osteogenic medium. ALP activity was detected histochemically. Non-MSC seeded HFF and OBC matrices were used as control. The results showed that HFF matrix did not suppress MSC osteogenesis, compared to OBC matrix. OBCs were derived from day-15 osteogenic culture of bone marrow derived MSCs from a 47-year-old female donor. (JPEG 637 kb)