Additional file 7: Figure S6. of CENH3-GFP: a visual marker for gametophytic and somatic ploidy determination in Arabidopsis thaliana

DAPI-stained chromosome spreads of 3x Arabidopsis male meiocytes. DAPI-stained chromosome spreads of somatic (A) and male meiotic (B, C and D) nuclei of triploid Arabidopsis thaliana plants (3x = 15). The somatic nucleus exhibits 15 distinct chromosomes, reflecting the triploid somatic chromosome number. Male meiotic chromosome spreads reveal the formation of five trivalents at diakinesis (B) and metaphase I (C), that inherently lead to an unbalanced segregation of homologous chromosomes at anaphase I (D; 8–7 chromosome segregation). Scale bar, 10 μm. (DOC 206 kb)