Additional file 7: Figure S3. of Intraperitoneally infused human mesenchymal stem cells form aggregates with mouse immune cells and attach to peritoneal organs

Production of mouse cytokines in the peritoneal cavity after MSC administration. Mouse cytokines were assayed in peritoneal lavage of MSC-injected BALB/c mice. Based on the timing of the cytokine production, they were categorized into (A) early- (peak production within 4 h), (B) intermediate- (peak production on day 3), or (C) late-responding cytokines (peak production beyond day 3). Data are representative of two independent experiments. Values are arithmetic means ± SEM, n = 4–6. (D) Relative gene expression of selected mouse cytokines in omentum (OM), mesentery (MT), peritoneal lavage pellet (PLP) and jejunal lymph nodes (JLN). Values are average gene expression values normalized to mouse Gapdh using 2–ΔCt method ± SEM, n = 4 to 5. *P < 0.05; **P < 0.01; ***P < 0.001 compared to baseline expression levels. (E) Relative gene expression of human cytokines in omentum, mesentery, peritoneal lavage pellet and jejunal lymph nodes. Values are fold change of gene expression in vivo over corresponding gene expression in unstimulated in vitro cultures of MSCs prior to the injection into mice represented on the graph by dotted line. The expression values were obtained using 2–ΔΔCt method with human GAPDH as housekeeping control. (PDF 105 kb)