Additional file 6: of Term amniotic fluid: an unexploited reserve of mesenchymal stromal cells for reprogramming and potential cell therapy applications

Showing TAF-iPS cell lines are pluripotent. (a) Q-RT-PCR analyses of OCT4, SOX2, KLF4, C-MYC, NANOG, DNMT3B, TDGF1 (CRIPTO), and ZFP42 (REX1) expression in nine TAF-iPS, CB-iPS and hES cell lines. Samples were normalized against the internal control (GAPDH) and plotted (log10 scale) relative to the expression level in the hES cell line HUES-3, which is arbitrarily set to a value of 1. (b) Histological analyses of teratomas generated by TAF-iPS cell lines reveal the presence of tissues with characteristic structures of all three germ layers. Representative images of haematoxylin and eosin (H&E)-stained teratoma sections, generated from a TAF-iPS cell line following subcutaneous injection of 500,000 iPS cells into NSG mice. (TIF 14420 kb)