Additional file 6: of Comprehensive profiling of functional Epstein-Barr virus miRNA expression in human cell lines

AKBM cells are EBV positive. A) Schematic representation of the EBV super-sensor. Five perfect EBV miRNA target sites were cloned downstream of the mCherry reporter gene. The vector allows for sensing of EBV presence by monitoring the combined functional expression of 5 EBV miRNAs. B) EBV-positive AKBM cells and EBV negative AK31 and 2A8 cells were either left untreated (cells alone), were transduced with an mCherry-control vector (control sensor), or were transduced with the EBV sensor from a). Cells were monitored for mCherry reporter expression at 7 days post transduction by flow cytometry (PDF 165 kb)