12864_2016_2978_MOESM6_ESM.pdf (165.98 kB)
Additional file 6: of Comprehensive profiling of functional Epstein-Barr virus miRNA expression in human cell lines
journal contribution
posted on 2016-08-17, 05:00 authored by Marjolein Hooykaas, Elisabeth Kruse, Emmanuel Wiertz, Robert LebbinkAKBM cells are EBV positive. A) Schematic representation of the EBV super-sensor. Five perfect EBV miRNA target sites were cloned downstream of the mCherry reporter gene. The vector allows for sensing of EBV presence by monitoring the combined functional expression of 5 EBV miRNAs. B) EBV-positive AKBM cells and EBV negative AK31 and 2A8 cells were either left untreated (cells alone), were transduced with an mCherry-control vector (control sensor), or were transduced with the EBV sensor from a). Cells were monitored for mCherry reporter expression at 7Â days post transduction by flow cytometry (PDF 165 kb)