Additional file 6: of A potential gliovascular mechanism for microglial activation: differential phenotypic switching of microglia by endothelium versus astrocytes

Figure S6. Microglia were subjected to OGD for 4 h, and then endo-CM or astro-CM were added into OGD-treated microglia for another 8 h (for gene expression experiment using real-time PCR) or 24 h (for ELISA measurement of cytokines). (a) Endo-CM treatment upregulated the expression of iNOS, whereas astro-CM treatment upregulated the expression of CD206. (b, c) Endo-CM significantly increased the release of TNFα and IL-10 from OGD-treated microglia. (PDF 104 kb)