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Additional file 6: Figure S6. of ULK1 phosphorylates Sec23A and mediates autophagy-induced inhibition of ER-to-Golgi traffic
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posted on 2017-05-10, 05:00 authored by Wenjia Gan, Caiyun Zhang, Ka Siu, Ayano Satoh, Julian Tanner, Sidney YuThe interactions between the ULK1 phosphorylation mutants of Sec23A and Sec31A. (A) S207 and T405 mutants of Sec23A were tested for their interaction with Sec31A by co-expressing the Myc-His-Sec23A mutants and GFP-Sec31A. (B) Combinations of S207 and T405 double mutants were tested for the interaction with Sec31A. Overexpression of the indicated protein was carried in HEK293T cells, followed by immunoprecipitation of Myc-His-Sec23A by anti-Myc antibody. Co-precipitated GFP-Sec31A was detected by anti-GFP antibody. (B) Wildtype Sec23A, S207A and T405A mutants were tested for the interaction with GFP-Sec31A in growth medium or in amino acid starved medium EBSS. (TIFF 5305Â kb)