13287_2017_764_MOESM5_ESM.doc (322 kB)
Additional file 5: of The role of fibrinolysis inhibition in engineered vascular networks derived from endothelial cells and adipose-derived stem cells
journal contribution
posted on 2018-02-12, 05:00 authored by Severin Mühleder, Karoline Pill, Mira Schaupper, Krystyna Labuda, Eleni Priglinger, Pablo Hofbauer, Verena Charwat, Uwe Marx, Heinz Redl, Wolfgang HolnthonerThrombin transiently activates EC. HUVEC-ASC clots were fixed and immunofluorescence was performed against E-Selectin after 4 h, 1 day, 4 days and 7 days. Staining revealed transient activation of EC via thrombin after 4 h. TNF-α (10 ng/ml), serving as a positive control, activated EC more strongly after 4 h than thrombin alone. No aprotinin was used in any sample. n = 8 from two independent experiments. Scale bar: 200 μm. (DOC 322 kb)
