Additional file 5: Figure S5. of Microglial depletion alters the brain neuroimmune response to acute binge ethanol withdrawal

Acute binge ethanol increases brain TNFα, Ccl2, and IL-4 protein during withdrawal. Mice were treated with ethanol (6 g/kg, 25% v/v) or water and sacrificed 18 h post-treatment. Brain protein was collected and A) TNFα, B) Ccl2, C) IL-1ra, and D) IL-4 protein levels were determined via ELISA. Data are represented as mean ± SEM. *p < 0.05, Student’s t test, n = 6/group (TIF 97 kb)