Additional file 5: Figure S5. of An improved pre-clinical patient-derived liquid xenograft mouse model for acute myeloid leukemia

Frequency of CD34+ AML cells is greater in newborn NSG pups than adult NSG mice. Magnetically sorted CD34+ pooled BM cells and splenocytes from secondary engrafted NSG mice were injected intrahepatically in newborn NSG pups (1Gy; n = 5) or intravenously in 6-week-old NSG adults (2.5Gy; n = 5) after sublethal irradiation (1 × 105 cells per adult or pup). a Representative flow cytometry plots illustrating the expression of CD34 and CD117 in human CD45 cells in peripheral blood, spleen, and BM of NSG pups and adult NSG mice at endpoint. b, c, and d Comparison of frequency of human CD45 relative to total CD45 b, total CD34+ c, and CD34+CD117+ d cells relative to human CD45 cells in peripheral blood, spleen, and BM between newborn NSG pups and adult NSG mice at endpoint (week 17–20 post-engraftment). Data are presented as mean frequencies ± SEM. Two-tailed Mann Whitney U test; *; p < 0.05. (TIFF 2359 kb)