Additional file 5: Figure S2. of Development of high-lysine rice via endosperm-specific expression of a foreign LYSINE RICH PROTEIN gene

Pollen fertility of PA110 and wild-type. (a) and (b) Pollen under the sterile condition. (c) and (d) Pollen under the fertile condition. Matured pollen was stained with 1 % potassium iodide, dark staining indicates viable pollen. Scale bars, 50 μm. (e) and (f) Fluorescent staining by 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) detected the nucleus status of pollen under fertile condition, light and faint staining indicate two sperm nucleus and vegetative nucleus, respectively. Scale bars, 5 μm. (JPG 5437 kb)