Additional file 4: of Morphological adaptation of sheep’s rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis

Figure S3. Cell apoptosis distribution in the ruminal epithelium of sheep fed purely hay (A: CON) and a high-grain diet for 7 (B: G7), 14 (C: G14) and 28 d (D: G28). Q4: Nonapoptotic, live cells do not bind Annexin V-FITC and exclude PI(Propidium lodide); Q3: Early apoptotic cells bind Annexin V-FITC and exclude PI; Q2: Late apoptotic cells bind Annexin V-FITC and also PI. Apoptosis cells = Q2 (Late apoptotic cells) + Q3 (Early apoptotic cells). (TIFF 1543 kb)