Additional file: 4 of Application of NeuroTrace staining in the fresh frozen brain samples to laser microdissection combined with quantitative RT-PCR analysis

Figure S4. Transcript levels of (A) 18S rRNA, (B) GAPDH, (C) β-actin, and (D) Map2 mRNAs in the hippocampal DG region from samples that were cryosectioned at a thickness of 20 μm, 30 μm, and 40 μm. Values are expressed as the copy number of genes per LMD tissue of 182 nm2 × cryosection thickness in volume. Bars indicate the mean ± SEM. Asterisks (*p < 0.05, **p < 0.01, ***p < 0.001) express statistically significant differences between fixed and stained specimens and the corresponding unfixed and unstained samples, as assessed by two-way ANOVA followed by Bonferroni post hoc test. For unfixed and unstained (Untreated) samples, n = 7 for 20 μm, n = 6 for 30 μm, and n = 8 for 40 μm; for fixed and stained samples, n = 7 for 20 μm, n = 7 for 30 μm, and n = 8 for 40 μm.