Additional file 4: Movie S2. of Growth of human breast tissues from patient cells in 3D hydrogel scaffolds

Time-lapse microscopy depicting a rotating lobule and the behavior of leader cells. The arrow indicates the direction of rotation of the lobule on the left. Arrowheads indicate the location of leader cells extending from two elongating outgrowths. At the outset of the movie, the organoid had been grown for 8 days. Images were captured every 10 minutes. Frame rate: eight frames per second. (AVI 9677 kb)