Additional file 4: Figure S4 of A brain-targeted, modified neurosin (kallikrein-6) reduces α-synuclein accumulation in a mouse model of multiple system atrophy

Levels of α-syn immunoreactivity in individual oligodendroglial cells in the brains of MBP-α-syn tg that received LV-NR-R80Q-apoB. Vibratome brain sections were analyzed by double immunofluorescence and confocal microscopy for analysis of the levels of human α-syn (red) in oligodendroglial cells labeled with an antibody against p25 (green). Dotted box to the left depicts the image field zoomed represente d under detail. (A) Laser scanning confocal microscopy of sections from non-tg and MBP-α-syn tg immunostained with human α-syn (red) and p25 (green). (B, C) Computer aided image analysis of p25 positive oligodendrocytes expressed as numbers per 103 in the striatum and of the levels of α-syn in individual p25 positive cells expressed as pixel intensity. n = 10 mice per group. Scale bar = 30 μm. (TIFF 2838 kb)