Additional file 4: Figure S3. of Point of sampling detection of Zika virus within a multiplexed kit capable of detecting dengue and chikungunya

Limit of detection for 1-plex chikungunya and dengue-1 RT-LAMP experiments. Substrates for this experiment were extracted viral RNA from Vero cell cultures. (A) Varying titers of chikungunya viral RNAs (~189 to 18 copies) were included in RT-LAMP reagents and run real-time using Light cycler (channel 523-568). For chikungunya detection, 80 nM of HEX-labeled probes were used, and about 38 copies of chikungunya viral RNA could be detected in less than 30 min. (B) Varying titers of dengue-1 viral RNAs (~2.44 to 0.12 pfu equivalent RNA copies) were included in RT-LAMP reagents and run real-time using Light cycler (channel 558-610). For dengue-1 detection, 80 nM of TAMRA-labeled probes were used, and about 1.22 pfu equivalent copies of dengue-1a viral RNA could be detected within 35 min (JPEG 58 kb)