Additional file 4: Figure S2. of Spatial sexual dimorphism of X and Y homolog gene expression in the human central nervous system during early male development

Combined padlock hybridization for PCDH11X and Y and Immunohistochemistry with Islet-1 antibodies in SC samples from human male embryos. a, b Padlock probe hybridization with probes for PCDH11X and PCDH11Y was combined with immunohistochemistry using islet-1 antibodies. Islet-1-positive cells are stained in yellow, PCDH11X signals are in sky blue and PCDH11Y in red. DAPI staining in dark blue allows visualization of nuclei. Part b is a detail enlargement of the complete SC section shown in a. (TIF 4288 kb)