Additional file 3: of Transcriptome analysis revealed that a quorum sensing system regulates the transfer of the pAt megaplasmid in Agrobacterium tumefaciens

Mutagenesis of the quorum sensing determinants of pAtP4. All mutations were generated by the insertion of the same Gm resistance cassette that originated from p34S-Gm [39] either at the XbaI site of the cinI gene or at a reconstituted SphI site generated by the fusion of parts of cinR and cinX determinants in a synthetic ORF. (PDF 46 kb)