Additional file 3: of Oxygen transfer rate identifies priming compounds in parsley cells

Reference cultivations for the Pep13-dose dependent responses of parsley cell suspension cultures. Oxygen transfer rate (OTR) as a function of time for three Pep13 concentrations: (A) addition of 100 pM Pep13, (B) addition of 50 pM Pep13, (C) addition of 1 pM Pep13. Cultivations: w/o additives (black squares), with the addition of exclusively 100 μM SA after 72 h (blue diamonds), with the addition of exclusively Pep13 after 96 h (gray up triangles), and with the addition of 100 μM SA and Pep13 (green circles). Dotted lines indicate the addition of (1) SA, (2) Pep13, and (3) sampling for the furanocoumarin fluorescence measurements. D-E: The experiments are compared at an excitation wavelength of 335 nm and an emission wavelength of 400 nm. Cultivations: w/o additives (black columns), with the addition of exclusively salicylic acid (SA) (blue columns), with the addition of exclusively 50 pM Pep13 (gray columns), and with the addition of 100 μM SA and 50 pM Pep13 (green columns). Cultivation conditions: 250 mL flask volume, 50 mL filling volume, 180 rpm shaking frequency, 5 cm shaking diameter, and 25 °C. (TIF 4013 kb)