Additional file 3: of Impact of flanking chromosomal sequences on localization and silencing by the human non-coding RNA XIST

Candidate gene silencing assays for each integration site. One assay for each integration site was included in Fig. 2. a Allelic pyrosequencing of three cDNAs from No DOX and DOX were compared in duplicate pyrosequencing reactions. As a control cDNA from a different integration was also assessed. b For the X-chromosome integration site q-RT-PCR was used to determine silencing as the cells are hemizygous. c Q-RT-PCR assays were also performed to validate some autosomal silencing and compare with pyrosequencing and RNA-seq. (PDF 933 kb)