Additional file 3: Figure S3. of Targeting mitochondrial complex I using BAY 87-2243 reduces melanoma tumor growth

Complex I inhibition causes oxidative stress and ROS-mediated cell death. (A) G-361 and SK-MEL-28 cells were treated with increasing concentrations of BAY 87-2243. Mitochondrial ROS levels were measured using the MitoSOX dye after 24 hours (n = 2). (B) A-375 cells were treated with BAY 87-2243 (10 nM) and the antioxidant, NAC (5 mM). Cytosolic ROS levels were measured using the redox-reactive dye CM-H2DCFDA after 24 h (n = 3). (C) Melanoma cells were treated with BAY 87-2243 (10 nM) and the antioxidant NAC (5 mM). Cell death was measured after 72 h using propidium iodine (n = 3). (D) G-361 cells were treated with BAY 87-2243 (10 nM, 100 nM) and the antioxidant, NAC (5 mM). Cell lysates were collected from treated G-361 cells (24 h) and probed with antibodies recognizing cleaved PARP. Actin was used as a loading control. Data are represented as the mean ± SD. *p < 0.05.