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Additional file 3: Figure S3. of E2-EPF UCP regulates stability and functions of missense mutant pVHL via ubiquitin mediated proteolysis

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posted on 2015-10-26, 05:00 authored by Kyeong-Su Park, Ju Kim, Hee Shin, Kyung-Sook Chung, Dong-Soo Im, Jung Lim, Cho-Rok Jung
Ubiquitination is decreased in lysine zero mutant pVHL in cells significantly. (C) Single-lysine (K159, K171, and K196) and lysine-zero mutant pVHLs were analyzed in vitro ubiquitination assay. GST-tagged VHL was incubated with E1, Flag-ubiquitin and His-UCP at 37 °C for 1 h. Each GST-VHL was pulled-down with glutathione sepharose beads and analyzed by anti-GST or anti-HA antibody. (TIFF 115 kb)

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Ministry of Education, Science and Technology

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