Additional file 3: Figure S1. of Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration

DPPSC characterisation. a-d Phase contrast images of DPPSC morphology in the primary culture (a), passage (P)5 (b), P10 (c) and P15 (d). Scale bars: 200 μm. e Population-doubling time in hours of DPPSC for 15 passages. n = 13 different donors, results are displayed as mean ± s.e.m.. f Number of divisions per passage of DPPSCs for 15 passages. n = 13 different donors, results are displayed as mean ± s.e.m. g Example of a short-Comparative Genomic Hybridisation in DPPSC at P15 showing no chromosomal abnormalities. The DNA control used for the hybridisation was XXY, therefore the observed loss of chromosome Y indicates these cells are from a female donor. h Relative mRNA fold change expression of the pluripotency markers OCT4A and NANOG at P5, P10 and P15 compared to P5 in DPPSC from eight different donors. ***p < 0.001, n = 8 different donors, two-way ANOVA was used and results are displayed as mean ± s.e.m. i, j Immunofluorescence analyses for the pluripotency markers NANOG (red; i) and SOX2 (red; j) in undifferentiated DPPSC at P10. Nuclei are counterstained with DAPI (blue). Merge is observed in purple. Scale bars: 100 μm. k Alkaline phosphatase (ALP) staining of DPPSC at P10. Scale bar: 100 μm. (PDF 196 kb)