Additional file 2: of Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology

Figure S2. (A) 293 T cells transfected with Flag-Znf179 or Flag-Znf179-5A mutant for 48 h were then treated with 10 μM MG132 or 10 mM 3-MA for 4 h. Total cell lysates were analyzed by Western blotting with anti-Znf179 and anti-β-actin antibodies. (B) Protein intensity were quantified by AlphaEase FC software and the statistic results were presented as mean ± s.e.m. of at least three independent experiments (*, P < 0.05). (TIF 833 kb)