Additional file 2: of TREM2 deficiency exacerbates tau pathology through dysregulated kinase signaling in a mouse model of tauopathy

Quantification strategy for p-tau + neurons. A Cresyl violet staining was utilized to determine specific laminar layers II-III and IV-VI which were used to define quantitation of p-Tau+ neurons. A total of 2- medial sections were analyzed per mouse (n = 4 per genotype). Individual AT8 and AT180 positive cell bodies were counted in layers II-III and layers IV-VI in hTau and hTau;Trem2 −/− mice. B No genotype specific differences in neurodegeneration were detected between hTau and hTau;Trem2 −/− mice at 6 months of age as measured by NeuN reactivity. (PDF 1673 kb)