Additional file 2: of Geminin prevents DNA damage in vagal neural crest cells to ensure normal enteric neurogenesis

Figure S1. Ablation of Gem from early pre-ENCCs leads to severe intestinal aganglionosis. Whole-mount gut preparations of control (A) and Wnt1Cre|Gem (B–C) E12.5 embryos immunostained for GFP to visualise migrating ENCCs. Red arrows indicate the position of the most caudally located ENCCs in the gut preparations. Scale bar: (A–C) 400 μm. (TIF 2895 kb)