13148_2019_738_MOESM2_ESM.pptx (474.16 kB)
Additional file 2. of Epigenetic down-regulation of the HIST1 locus predicts better prognosis in acute myeloid leukemia with NPM1 mutation
presentation
posted on 2019-10-13, 12:34 authored by Sylvain Garciaz, Lia N’guyen Dasi, Pascal Finetti, Christine Chevalier, Julien Vernerey, Mathilde Poplineau, Nadine Platet, Stéphane Audebert, Matthieu Pophillat, Luc Camoin, François Bertucci, Boris Calmels, Christian Récher, Daniel Birnbaum, Christian Chabannon, Norbert Vey, Estelle DuprezFigure S1. Related to Fig. 2: Heatmap of the H3K27me3 level in a new cohort of 78 NPM1mut CN-AML patients. Figure S2. Related to Fig. 2: Analysis of H3K27me3 HIST1 status in CD34low and CD34high sorted blasts. Figure S3 Related to Fig. 4. Representative Integrative Genomics Viewer (IGV) tracks of H3K27me3 signal obtained from ChIP-chip data published in Tiberi et al., 2015 Figure S4. Related to Fig. 5: Histone protein extraction in NPM1mut patients. Figure S5. Related to Fig. 5:Total protein abundance of each histone types determined by IBAQ label-free quantification method. Figure S6 related to Fig. 6. Effect of H1-3 KD on histone H1 subtype mRNA and protein expression. Figure S7 related to Fig. 6. Effect of H1d KD on CD11b expression in shRNA1 (clones KD#2 and KD#3) and in shRNA2 conditions. Figure S7 related to Figure 6. Effect of H1d KD on CD11b expression in shRNA1 (clones KD#2 and KD#3) and in shRNA2 conditions
