13395_2016_116_MOESM2_ESM.pptx (305.13 kB)
Additional file 2: Figure S2. of Laminin 521 maintains differentiation potential of mouse and human satellite cell-derived myoblasts during long-term culture expansion
presentation
posted on 2016-12-13, 05:00 authored by Christopher Penton, Vasudeo Badarinarayana, Joy Prisco, Elaine Powers, Mark Pincus, Ronald Allen, Paul AugustFACS sort of DBA/2J satellite cells. Cells were sorted for granularity (SSC × FSC), singlet selection (FSC-W × FSC-H, SSC-W × SSC-H), live/dead (propidium iodide), CD31−/CD45− (Percp-eFluor710), Pdgfrα−/Sca1− (BV421/BV605), and integrin α7+ (PE).
