Additional file 2: Figure S2. of Investigating the physiology of viable but non-culturable bacteria by microfluidics and time-lapse microscopy

Bulk and single-cell persister enumeration. Dependence of the frequency of persister cells on ampicillin concentration as measured via the single-cell microfluidics-microscopy assay (full circles) illustrated in Fig. 1 and the colony forming unit assay (open circles). The two assays are performed on aliquots withdrawn from the same E. coli overnight culture. Data and error bars are mean and standard error of the mean of measurements obtained in biological triplicate (N = 3). Data agreement within experimental error confirms the validity of the newly developed microfluidic assay. (PNG 226 kb)