Additional file 2: Figure S2. of Imaging the mammary gland and mammary tumours in 3D: optical tissue clearing and immunofluorescence methods

3DISCO clearing and 3D imaging of virgin and lactating mouse mammary tissue. a 3DISCO tissue clearing and immunostaining protocol and timeline. b Transmission images of 3DISCO cleared tissue. c Volume changes caused by 3DISCO-based clearing of virgin and lactating mammary tissue. Values are representative of measurements from three tissue pieces at each developmental timepoint. d 3D confocal imaging of 3DISCO-cleared virgin and lactating mammary glands immunostained with the basal cellb marker SMA. These images are representative of images from more than two mice. See Additional file 18 for a high resolution version of these PDFs. (PDF 8 mb)