Additional file 2: Figure S2. of Cooperative antitumor activities of carnosic acid and Trastuzumab in ERBB2+ breast cancer cells

CA inhibits ERBB2− in vitro breast cancer cell survival and migration. MCF7 and MDA-MB-231 cells were cultured for 10 days with control medium (Ctr), CA, Tz or CA + Tz supplemented medium, which was changed every 48 h. a Cell survival is expressed as arbitrary units (A.U.) after exposure to MTT agent for 4 h. Polynomial (Poly.) interpolation curves are shown. Mean values and standard deviation (indicated as vertical bars) from three independent replicates are shown. b MDA-MB-231 cells were cultured after overnight starvation for 7d with control medium (Ctr) or CA supplemented medium in the lower migration chamber, which was changed every 48 h. Representative images of the lower side of the migration membranes stained with Crystal Violet. Bar = 100 μm. c Relative cell migration expressed as percentage (%) compared to control was determined as detailed in Methods section. Mean values and standard deviation (indicated as vertical bars) from four independent replicates (n = 4) are shown. P < 0.001 (***) (TIFF 10150 kb)