Additional file 2: Figure S1. of Positive geotactic behaviors induced by geomagnetic field in Drosophila

Near-zero GMF-induced geotactic response under different ZTs and the comparison of vertical choice vs. horizontal choice Y-maze assays. (A) Photos of the test cube (180 × 100 × 140 mm, length × width × height) used for the tube-positioning assay. (B) Comparisons of the geotactic positioning of wild-type flies under the sham and cancellation conditions during ZT0 to ZT2 (n = 10 trials). Error bars: SEM. *, P < 0.05 by Student’s t-test. (C) Photos of the double-layered permalloy (0.5 mm thick) cube (180 × 100 × 140 mm, length × width × height) used for the attenuation of GMF by passive cancellation. (D) Comparisons of the geotactic positioning of wild-type flies under the sham and passive cancellation conditions during ZT5 to ZT8 (n = 10 trials). Error bars: SEM. *, P < 0.05 by Student’s t-test. (E) Comparisons of the exit profiles of wild-type flies making vertical choice versus horizontal choice in the Y-maze assays under the sham condition (n = 12 trials). Error bars: SEM. *, P < 0.05; **, P < 0.01 by Student’s t-test. (PDF 81 kb)