Additional file 2: Additional Figure S2. of The role of the aryl hydrocarbon receptor in the development of cells with the molecular and functional characteristics of cancer stem-like cells

AHR modulation alters ALDH activity in ER−, immortalized MCF-10 F epithelial cells. (A) Representative flow cytometry dot plots of ALDEFLUOR™ staining of MCF10F cells treated for 48 hours with vehicle, 10 μM CH223191, or 0.5 μM FICZ. Regions representing ALDHhigh cells were drawn based on the signal generated in the presence of DEAB. (B) MCF-10 F cells were treated as in (A) and assayed for the percentage of ALDHhigh cells. Data from four experiments were normalized to results obtained with naive cells (mean baseline = 0.2 % ALDHhigh cells) and presented as mean fold-change from naive ± standard error. Asterisks indicate a significant decrease in the percentage of ALDHhigh cells, *P <0.05, **P <0.005. A cross indicates a significant increase in ALDHhigh cells, + P <0.05. (PDF 97 kb)