Additional file 1: of YKL-40 (Chitinase 3-like I) is expressed in a subset of astrocytes in Alzheimer’s disease and other tauopathies

Figure S1. Semi-automated method for pathological burden quantification. For all conditions tau and GFAP were assessed using a randomized computer-based quantification of patterns and severity in immunohistochemical stains. Cortical grey matter of each case was delimited blinded to clinical phenotypes (A). We developed an in-house algorithm that allows defining randomized regions of interest (ROIs) on a full-section scan (B–C), to compute density of protein expression (D) and to quantify the number of pathological objects (E). (TIF 4423 kb)