Additional file 1: of Rapid and efficient generation of neural progenitors from adult bone marrow stromal cells by hypoxic preconditioning

Figure S1. Work flow for the derivation of fate-committed Schwann cells from BMSCs. BMSCs isolated by means of adherence to tissue culture plastic were dissociated and maintained for 12 days in the presence of EGF/bFGF on low-attachment culture plates. Thereafter, resultant neurospheres were plated onto PDL/laminin-coated culture plates and differentiated into Schwann cell-like cells (SCLCs) in glial differentiation medium containing β-heregulin, bFGF, and PDGF for 7 days. In order to direct SCLCs to fate commitment, they were co-cultured with purified and partially dissociated dorsal root ganglia (DRG) neurons for a further 15 days. (PDF 159 kb)