Additional file 1: of Morphological adaptation of sheep’s rumen epithelium to high-grain diet entails alteration in the expression of genes involved in cell cycle regulation, cell proliferation and apoptosis

Figure S1. Rumen papillae histology (scale bar = 100 μm) of hay-fed (A: CON) and high-grain diet-fed sheep (B: G7; C: G14; D: G28). (SC, stratum corneum; SG, stratum granulosum; SS, stratum spinosum; SB, stratum basale). Sheep assigned to CON (n = 5), G7 (n = 5), G14 (n = 5) and G28 (n = 5) received a high-grain diet for 0, 7, 14 and 28 d, respectively. The stratum corneum (SC) was the outermost cell layer, which was heavily stained and the stratum granulosum (SG) was defined as the layer of long axes cells which lay perpendicular to the stratum spinosum (SS) and basale (SB). SS and SB were cells nested between the lamina propria and SG. (TIFF 6443 kb)