12943_2020_1201_MOESM1_ESM.pdf (723.71 kB)
Additional file 1 of Long noncoding RNA ZFAS1 promoting small nucleolar RNA-mediated 2′-O-methylation via NOP58 recruitment in colorectal cancer
journal contribution
posted on 2020-05-23, 03:31 authored by Huizhe Wu, Wenyan Qin, Senxu Lu, Xiufang Wang, Jing Zhang, Tong Sun, Xiaoyun Hu, Yalun Li, Qiuchen Chen, Yuanhe Wang, Haishan Zhao, Haiyan Piao, Rui Zhang, Minjie WeiAdditional file 1: Table S1.Short hairpin RNAs (shRNAs) sequence against ZFAS1. Table S2.Short hairpin RNAs (shRNAs) sequence against NOP58. Table S3. The reaction assays of qRT-PCR method. Table S4. Primers used in qRT-PCR assays. Table S5. Probes used in situ hybridization (ISH) assay. Table S6. RNA probes used for RNA pull-down assays. Table S7. Primers used in site-specific 2′-O-methylation for RTL-P assay. Table S8. RTL-P assays for rRNA 2′-O-methylation. Table S9. Probes used in site-specific 2′-O-methylation for double-stranded primer based on single-stranded toehold (DPBST) assays. Table S10. Double-stranded primer based on single-stranded toehold (DPBST) assays. Table S11. Data of LncRNAs cluster in Heat map analysis. Table S12. Data of snoRNAs cluster in Heat map analysis. Table S13. Data of mRNAs cluster in Heat map analysis. Table S14. The enriched target genes intersected by ZFAS1, SNORD12C/78 in GO analysis. Table S15. Prognostic information of included colorectal cancer patients (n = 157). Table S16. Multivariate COX regression analysis of the association of ZFAS1 and NOP58 expression with DFS and OS in CRC patients.
