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Additional file 1: of Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis

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posted on 2017-05-16, 05:00 authored by Shinichiro Nakachi, Shuji Sumitomo, Yumi Tsuchida, Haruka Tsuchiya, Masanori Kono, Rika Kato, Keiichi Sakurai, Norio Hanata, Yasuo Nagafuchi, Shoko Tateishi, Hiroko Kanda, Tomohisa Okamura, Kazuhiko Yamamoto, Keishi Fujio
Supplementary methods and figures. Figure S1 Expression of FOXP3 in LAG3+ Tregs and CD25+ Tregs. Freshly isolated human CD4+ T cells were stained for FOXP3 and LAG3. Representative FACS data from three independent experiments are shown. Figure S2 In vitro T-cell proliferation assay. Carboxyfluorescein succinimidyl ester-labeled naive T cells were cultured with irradiated APCs and CD25+ Tregs for 72 h. Representative FACS data from three independent experiments are shown. Figure S3 Frequencies of IL-10+ cells in 7-AAD− cells detected in intracellular staining of naive CD4+ T cells and LAG3+ Tregs (n = 4). * P < 0.05 by Mann-Whitney U test. Figure S4 Scatterplot of frequencies of CD25+ Tregs or LAG3+ Tregs vs ACPA titer or RF (n = 83). P value is for Spearman’s rank correlation coefficient. rho Spearman’s rho. Linear regression line is drawn as a blue line, and 95% CI region is presented as a gray area. a Scatterplot of frequencies of CD25+ Tregs vs ACPA titer. b Scatterplot of frequencies of CD25+ Tregs vs RF titer. c Scatterplot of frequencies of LAG3+ Tregs vs ACPA titer. d Scatterplot of frequencies of LAG3+ Tregs vs RF titer. Figure S5 Changes in percentages of LAG3+ Tregs in CD4+ T cells (ΔLAG3) were evaluated in accordance with treatment response to abatacept (n = 18). Patients were divided into three groups (no response, moderate response, and good response) following European League Against Rheumatism response criteria based on DAS28-ESR. * P < 0.05 by Kruskal-Wallis test and Dunn’s multiple-comparisons test. (ZIP 963 kb)

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Japan Society for the Promotion of Science

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    Arthritis Research & Therapy

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