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Additional file 1: of Implementation and application of a multiplex assay to detect malaria-specific antibodies: a promising tool for assessing malaria transmission in Southeast Asian pre-elimination areas

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posted on 2015-09-04, 05:00 authored by Karen Kerkhof, Lydie Canier, Saorin Kim, Somony Heng, Tho Sochantha, Siv Sovannaroth, Inès Vigan-Womas, Marc Coosemans, Vincent Sluydts, Didier MÊnard, Lies Durnez
Example of Levey Jenning Charts plotted for the quality control of the immunoassay used for screening the field bloodspot samples. Data analysis started with a quality control on the ΔMFI-values of the 100 % positive control pool samples (A). The dots represent each positive control sera sample in duplicate per plate. If these dots fell out of the -2SD and +2SD (red area), these plates were rejected and re-analysed. The same quality control was also performed on the PP calculated from the 50 % positive control pool samples per Ag (B). Based on the outcome of both graphs, plates were accepted or rejected and reanalysed.

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