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Additional file 1: of HDL in diabetic nephropathy has less effect in endothelial repairing than diabetes without complications

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posted on 2016-04-14, 05:00 authored by Yufeng Li, Mingming Zhao, Dan He, Xuyang Zhao, Wenjing Zhang, Lixin Wei, Edgar Huang, Liang Ji, Meng Zhang, Belinda Willard, Zuodi Fu, Lijuan Wang, Bing Pan, Lemin Zheng, Linong Ji
Supplement Figure 1: Diabetic and diabetic nephropathic high-density lipoprotein is less efficient in stimulating EC proliferation. HUVECs were treated with N-HDL, D-HDL and DN-HDL for 24 hours, and cell proliferation was measured using Brdu assay. (Mean + SD, n = 6, *p<0.05; ***p<0.001; one-way ANOVA). Supplement Figure 2: Glycated high-density lipoprotein in vitro have a higher glycation level and a reduced capability to promote EC migration compared with normal and diabetic HDL. A: Normal HDL was incubated in 25 mM glucose solution at 37 °C over 7 days and HDL glycation level was measured using MS. B: HUVEC monolayers were scratched by manual scraping and treated with PBS, N-HDL, D-HDL, DN-HDL or G-HDL respectively at 100 ug/ml apoA-I for 10 hours. Migration into the wound was photographed (50X objective lens). Distance between gaps was measured, and the results were expressed as percentage of various HDL-treated cells in comparison with control. C: HUVECs were treated with PBS, N-HDL, D-HDL, DN-HDL or G-HDL at 100 ug/ml apoA-I for 10 hours respectively in transwell assay, and pictures were taken in 6 random high-power (50X) fields. Migratory cells were counted, and the results were expressed as percentage of various HDL-treated cells in comparison with control (*p<0.05; **p<0.01; ***p<0.001; one-way ANOVA). (DOCX 996 kb)

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