Additional file 1: Table S1. of Hox genes reveal genomic DNA variation in tetraploid hybrids derived from Carassius auratus red var. (female) × Megalobrama amblycephala (male)

The PCR Gene-specific degenerate primers. Gene-specific degenerate primers designed based on the alignment and identification of consensus orthologous Hox gene sequences from zebrafish (Danio rerio), medaka (Oryzias latipes), pufferfish (Fugu rubripes), mouse (Mus musculus), cichlids, and humans (Homo sapiens). Table S2. The Percentage of the amino acid. Percentage amino acid identity between paralogous Hox sequences obtained from 4nF1 and reported orthologues from zebrafish, fugu, and medaka. Table S3. Comparison of GC levels among duplicated genes. (DOCX 20 kb)